Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)) is a truncated peptide derived from the human Myelin Oligodendrocyte Glycoprotein (MOG).

CD8CD3和CD4CD3细胞经过CD8/CD4珠子富集后，分别占总CD3细胞的86%和94%。这些细胞被MOG35-55肽激活，并应用于增殖实验。尽管CD8 T细胞对MOG35-55的反应通常低于CD4 T细胞，但CD8 T细胞的反应并不总是依赖于CD4。与CD4 T细胞相比，CD8 T细胞分泌的IFN-γ和IL-4较少[1]。

采用野生型B6幼稚小鼠，通过将MOG35-55特异性T细胞从主动诱导EAE(aEAE)的B6小鼠中采集并移植至其体内，从而在这些小鼠中诱发了EAE。研究发现，与移植CD4 T细胞相比，从免疫B6小鼠中转移CD8 T细胞至幼稚B6小鼠后，后者显示出了类似的EAE评分以及轻微的炎症和脱髓鞘现象[1]。

Female C57BL/6 mice (n?=?20) were induced by myelin oligodendrocyte glycoprotein (MOG)35-55 peptide.?At 14 days after immunization, T cells were isolated from the spleen and purified as CD4 and CD8 T cells by using CD4 and CD8 isolation kits, and then the purity was determined by flow cytometric analysis.?These cells were stimulated by MOG35-55 peptide and applied to proliferation assays.?The interferon-gamma (IFN-γ) and interleukin (IL)-4 secretion of supernatant of cultured CD4 and CD8 T cells were measured by enzyme-linked immunosorbent assays (ELISA).?For adoptive transfer, recipient mice were injected with MOG35-55-specific CD8 or CD4 T cells.?EAE clinical course was measured by EAE score at 0-5 scale and spinal cord was examined by staining with hematoxylin and eosin and Luxol fast blue staining[1].